By M. Cristina Vega
This booklet offers complicated expression applied sciences for the construction of protein complexes. for the reason that complexes lie on the center of recent biology, the expression, purification, and characterization of enormous quantities of top of the range protein complexes is important for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to complicated reconstitution from person subunits, this booklet bargains precious insights and assistance for profitable protein expressionists.
Across a number of sections readers will observe current possibilities for the creation of protein complexes in bacterial structures (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, crops and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary approach. a final part introduces in short the various biophysical and structural characterization concepts for macromolecular complexes utilizing cutting-edge resolution scattering and nuclear magnetic resonance.
This paintings is a guided travel over one of the most robust and winning protein expression applied sciences, with a spotlight on co-expression and high-throughput functions. it really is addressed to every body drawn to the construction and characterization of macromolecular complexes, from college scholars who wish an available description of the main co-expression platforms to researchers in biomedicine and the lifestyles sciences looking for an updated survey of accessible technologies.
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Additional info for Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology)
Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega